Loss of Deacetylation Enzymes Hdac6 and Sirt2 Promotes Acetylation of Cytoplasmic Tubulin, but Suppresses Axonemal Acetylation in Zebrafish Cilia

Cilia are evolutionarily highly conserved organelles with important functions in many organs. The extracellular component of the cilium protruding from plasma membrane comprises an axoneme composed microtubule doublets, arranged a 9 + 0 conformation primary cilia or 2 motile cilia. These microtubules facilitate transport intraflagellar cargoes along axoneme. They also provide structural stability to cilium, which may play role sensory cilia, where signals received movement fluid. Post-translational modification is well-studied phenomenon, and acetylation on lysine 40 (K40) alpha tubulin prominent It believed that this contributes stabilization Two classes enzymes, histone acetyltransferases deacetylases, mediate regulation acetylation. Here we use genetic approach, immunocytochemistry behavioral tests investigate function deacetylases zebrafish model. By mutating three deacetylase genes ( Sirt2 , Hdac6 Hdac10 ), identify unforeseen for As expected, mutation these leads increased cytoplasmic tubulin, however, surprisingly it caused decreased developing eye, ear, brain kidney. ear eye showed elevated levels mono-glycylated suggesting compensatory mechanism. changes did not affect length morphology functional defects balance was observed, level cilium.